Method
The Method
How we select and grade the scientific literature.
Question identification
We start with a clinically relevant question: does this molecule have measurable effects on human health outcomes? We exclude in vitro and animal studies as primary evidence.
Systematic PubMed search
We use PubMed (NCBI) as our primary source. Filters applied: human studies, peer-reviewed, last 10 years (except foundational literature), study types: RCT > observational > case series. Each analysis reports the PMIDs of main sources.
Evidence grading (A–F)
We adapt the GRADE schema: A = Multiple high-quality RCTs, consistent effects B = Limited RCTs or moderate heterogeneity C = Good quality observational studies D = Weak or inconsistent evidence E = Only preliminary / animal studies F = No evidence or contradictory evidence
Conflict of interest disclosure
Authors disclose any economic interests related to the molecules discussed. Affiliate links are explicitly marked. Editorial ratings are independent of commercial relationships.
Periodic updates
Longevity literature evolves rapidly. Each analysis includes a last-revised date. Molecule pages are updated when significant new RCTs are published.
About
Nucleo editorial team has backgrounds in biochemistry, medicine and science communication. To preserve editorial independence, we maintain author anonymity. Each analysis is reviewed by at least two team members before publication.